Which of the Following Best Lists the Correct Order of the Steps

question 17

Multiple Choice

Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s?
I. Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading fra resulting in a fusion protein for each chain.
II. Disulfide bonds were induced to form between the cysteine residues of the purified insulin A a chains.
III. Both human insulin genes were reverse translated to create synthetic genes.
IV. EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional cloning of each into a separate plasmid vector.

• A) III, I, II, IV
• B) I, II, III, IV
• C) I, III, IV, II
• D) III, IV, I, II
• E) IV, III, I, II

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Definitions:

Bipolar Disorder

A mental health condition characterized by extreme mood swings including emotional highs (mania or hypomania) and lows (depression).

Genetic Component

The part of a characteristic, condition, or disease that is influenced by genes inherited from one's parents.

Genetic Research

The scientific study focused on understanding the structure, function, evolution, mapping, and editing of genes.

Depression

An emotional well-being disorder identified by a constant feeling of sadness or a disinterest in usual activities, leading to major disturbances in daily functioning.