Which Lists the Potential Steps for Genetic Therapy for Mice

question 47

Multiple Choice

Which lists the potential steps for genetic therapy for mice with sickle cell disease (fiS/fiS) in the correct order? I. Differentiation of iPS cells into hematopoietic progenitor (HP) cells by infecting with retrovirus expressing HoxB4.
II. Reprogramming tail fibroblasts into induced pluripotent stem (iPS) cells by infecting them with vectors containing transcription factors.
III. Infection with a viral vector expressing Cre recombinase to remove transcription factor(s) from f iPS cells.
IV. Transplantation of corrected (þA/þA) hematopoietic cells back into irradiated mice.
V. Homologous recombination with fiS and wild- type fiA allele via CRISPR- Cas9 genome editing

• A) V, III, IV, I, II
• B) V, III, I, IV, II
• C) I, II, III, V, IV
• D) II, III, V, I, IV
• E) II, III, IV, V, I

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Definitions:

Confidence Interval

A span of numerical values, calculated from sample data, speculated to potentially contain the value of an unknown population parameter.

Population Proportion

The portion of a population that possesses a certain property or characteristic.

Drowsiness

A state of feeling abnormally sleepy or tired, often a symptom of a condition or result from certain medications, substances, or health issues.

Error of Estimation

The difference between an estimated value and the true value, reflecting the degree to which an estimation method may deviate from accuracy.