Your Student Is Trying to Create a "Gene X" Knockout

question 28

Essay

Your student is trying to create a "Gene X" knockout mouse by introducing a recombinant construct into embryonic stem (ES) cells, selecting transformants, and introducing the transformed cells into mouse blastocysts. He designed his construct as follows:
NeoR---- region of homology to Gene X--- HSV tk- -- region of homology to Gene X where NeoR is the neomycin- resistance gene and HSV tk is the Herpes Simplex virus thymidine kinase gene. He transforms the ES cells and exposes them to G418 and ganciclovir. Explain to your student he failed to recover transformed ES cells.

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Definitions:

Supply Chain Software

Computer programs designed to manage and optimize the logistics operations and processes within a supply chain, from procurement to delivery.

Real Time

Referring to processes or systems that can instantly respond and update based on inputs at the moment they are received.

Inventory Visibility

The ability of a company to track and understand its inventory levels, location, and status in real-time or near-real-time.

Integrated Supply Chain

A strategic approach to synchronizing the production, shipment, and delivery of products and materials in the most efficient and cost-effective way.