Which of the Following Best Lists the Correct Order of the Steps

question 17

Multiple Choice

Which of the following best lists the correct order of the steps taken to produce human insulin in E.coli in the 1970s?
I. Each insulin chain was cloned into an expression vector as a continuation of the lacZ reading fra resulting in a fusion protein for each chain.
II. Disulfide bonds were induced to form between the cysteine residues of the purified insulin A a chains.
III. Both human insulin genes were reverse translated to create synthetic genes.
IV. EcoRI and BamHI restriction sites were added to the ends of the DNA to facilitate directional cloning of each into a separate plasmid vector.

• A) III, I, II, IV
• B) I, II, III, IV
• C) I, III, IV, II
• D) III, IV, I, II
• E) IV, III, I, II

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Definitions:

Initial Assessment

The first evaluation or analysis of a patient or situation to determine the appropriate course of action or diagnosis.

Least Restrictive Intervention

An approach that involves using the most minimally invasive or limiting method necessary to achieve a desired outcome, especially in healthcare or education.

Behavioral Crisis

A situation where an individual's behavior poses an immediate danger to themselves or others, requiring urgent intervention.

Primary

Referring to the first or most significant stage, condition, or rank.