You Have Isolated a Plasmid from E Draw a Restriction Map of the Plasmid

question 15

Short Answer

You have isolated a plasmid from E. coli. You get the following results, using three restriction enzymes, 1 XhoI, 2 BamHI, and 3 EcoRI to perform six different digestions: single digests using each enzyme alone and double diges using each combination of two enzymes. $$\begin{array} { | l | l | l | } \hline \text { enzyme } & \text { \# fragments } & \text { sizes (in kb) } \\\hline \text { XhaI } & 1 & 10 \\\hline \text { BamHI } & 2 & 3.75,6.25 \\\hline E _ { c a R I } & 2 & 4.25,5.75 \\\hline X h a I + B a m H I & 3 & 1.25,3.75,5 \\\hline \text { XhaI } + E _ { c a R I } & 3 & 2,3.75,4.25 \\\hline \text { BamHI } + E _ { c o R I } & 4 & 1.25,2.5,3,3.25 \\\hline\end{array}$$ Draw a restriction map of the plasmid.

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