A Gene, VTE4, from the Sunflower of Known Sequence and Well-

question 24

Multiple Choice

A gene, VTE4, from the sunflower of known sequence and well- understood transcriptional regulation, is available from another lab. The product of this gene enables sunflowers to to produce a significantly increased amount of vitamin E from a substrate produced in all plants. The plant Brassica napus is harvested for canola oil. To produce canola oil with increased vitamin E, how would you proceed?

• A) Clone VTE4 in A. tumefaciens and use these bacteria to produce transgenic Brassica.
• B) Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.
• C) Introduce the gene and necessary cis elements into Brassica, along with a reporter gene, then measure the vitamin E produced.
• D) Introduce VTE4 into tomato plants or another food that humans eat more than Brassica.
• E) Select Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.

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