You Expect That a Mutation in E

question 30

Multiple Choice

You expect that a mutation in E. coli that was recently isolated results in loss of enzyme R activity. In looking for the gene you find comparable sequences. Using an in vitro transcription translation system, you use the gene obtained from the mutant as well as a wild type E. coli as a control. Following protein isolation and gel electrophoresis, you observe that the wild type E. coli produces a protein that is 35K in size, corresponding to enzyme R. However, the mutant strain produces a protein that is 25K in size. What does this information suggest?

• A)A missense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.
• B)A nonsense mutation has occurred, resulting in a truncated, nonfunctional product in the mutant.
• C)A large addition has occurred in the wild type strain, such that approximately 35 tandem repeats of this gene are present and are biologically active.
• D)all of the above
• E)Not enough information is given.

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Definitions:

Converging

The process of coming together or uniting in agreement or similarity, often used in the context of ideas, theories, or physical entities.

Double Image

A visual effect in which the same object is perceived twice, either due to optical reasons or conditions affecting the viewer's perception.

Autokinetic Effect

The autokinetic effect is a visual phenomenon in which a stationary, small point of light in an otherwise dark or featureless environment appears to move.

Stationary Light

A fixed light source that does not move, used in various contexts from photography to safety signaling.