You Are Attempting to Clone Your First Gene

question 24

Multiple Choice

You are attempting to clone your first gene. Up to the point of screening, everything has gone well. The vector has a gene for ampicillin resistance. In addition to the gene DNA, the insert also carries a gene for streptomycin resistance. Based on restriction digest, if properly inserted into the vector, your inserted DNA should be located within the ampicillin gene. Therefore, when screening you should look for clones that are

• A)ampicillin resistant and streptomycin sensitive.
• B)ampicillin resistant and streptomycin resistant.
• C)ampicillin sensitive and streptomycin resistant.
• D)ampicillin sensitive and streptomycin sensitive.
• E)Not enough information is given to determine the answer.

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Definitions:

Sound-on-film

A technology that synchronizes audio with moving pictures, allowing for sound to be recorded onto the same film strip as the image.

Movietone

Movietone refers to an early sound film system that combined motion pictures with synchronized recorded sound.

Three-strip Technicolor

Three-strip Technicolor is a color film process that uses three strips of film to capture the red, green, and blue color components, introduced in the 1930s to create vivid color movies.

Sound Cinema Technology

The technology enabling the synchronization of motion picture with sound, revolutionizing the film industry in the late 1920s.