In the Sanger Method of DNA Sequencing, Incorporation of the Nucleotides

question 29

Multiple Choice

In the Sanger method of DNA sequencing, incorporation of the nucleotides stops because

• A)the dideoxynucleotide analogs have no 2'- OH group to continue DNA synthesis.
• B)the dideoxynucleotide analogs have no 3'- OH group to continue DNA synthesis.
• C)the mixture is heated to 92°C to stop DNA synthesis at specific time intervals.
• D)the dideoxynucleotide analogs are in greater concentration than the actual nucleotides.
• E)the dideoxynucleotide analogs have no 5'- OH group to continue DNA synthesis.

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