A Variety of E

question 23

Multiple Choice

A variety of E. coli clones have been isolated, the DNA has been extracted, digested with restriction enzymes, denatured, separated by gel electrophoresis, and transferred to nylon membrane by the Southern blot procedure. A radioactive probe is used that hybridizes with a gene of interest. The DNA corresponding to a single clone yields a single DNA band after probing the blot. Based on this information only,

• A)the gene of interest is located entirely on the DNA band visualized.
• B)only the one clone described has the gene of interest.
• C)all of the clones tested, except the one described, have the gene of interest.
• D)the part of the gene recognized by the probe is located on the DNA band visualized.
• E)both B and D

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Definitions:

Nominal

Relating to variables that consist of categories or labels with no inherent order or ranking among them.

Gender of Child

A demographic variable indicating the biological sex of a child.

Samples and Populations

Refers to the process of selecting a subset (sample) from a larger group (population) to make inferences about the whole.

Nominal

Pertaining to data that consists of names, labels, or categories without any inherent order or ranking.