Say You Were Using Random PCR Primers to Amplify Fragments

question 6

Essay

Say you were using random PCR primers to amplify fragments of DNA. Would you expect to get more or less amplification products if you set the PCR machine at a relatively higher or lower annealing temperature, respectively, and why?

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Definitions:

Mean

The mean is the average of a set of numbers, calculated by dividing the sum of all values by the number of values.

Standard Deviations

Measures that express the variability or spread in a set of data from its mean, showing how much variation there is from the average.

Normal Curve

A symmetrical, bell-shaped curve representing the distribution of a set of data where most occurrences take place in the middle of the range and taper off toward either end.

Z Score

A statistical measurement that describes a value's relationship to the mean of a group of values, expressed in terms of standard deviations from the mean.