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The Following PH Dependence Was Found for the Activity of a Certain

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The following pH dependence was found for the activity of a certain enzyme-catalyzed reaction. The following pH dependence was found for the activity of a certain enzyme-catalyzed reaction.   If it is known that the only two ionizable residues in the active site are both glutamates, which conclusion can be drawn? A)  The glutamates have different microenvironments which cause their pK<sub>a</sub>ʹs to differ. B)  One of the glutamates must be amidated. C)  Both glutamates have a pK<sub>a</sub><sub> </sub>equal to 5.0. D)  Both glutamates are deprotonated during the reaction. If it is known that the only two ionizable residues in the active site are both glutamates, which conclusion can be drawn?


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