Mechanical Shearing Is Often the Preferred Method of Fragmenting Genomic

question 64

Multiple Choice

Mechanical shearing is often the preferred method of fragmenting genomic DNA when constructing a genomic DNA library. Why would this method be preferred over complete digestion with restriction enzymes?

• A) Enzymes might produce fragments that are too small or too large to be cloned.
• B) Enzyme cleavage can interrupt important gene sequences, in which case not all genes may be represented in the library.
• C) Shearing ensures the library contains overlapping fragments of DNA, which makes it possible to perform a chromosome walk.
• D) All of the above

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