The Primer Used in Sanger (Dideoxy Chain Termination) Sequencing

question 25

Multiple Choice

The primer used in Sanger (dideoxy chain termination) sequencing

• A) can have any random nucleotide sequence.
• B) has a nucleotide sequence complementary to the 5' end or the 3' end of the region to be copied.
• C) has a nucleotide sequence complementary to the 5' end of the region to be copied.
• D) must have a sequence beginning and ending with the same nucleotide.
• E) has a nucleotide sequence complementary to the 3' end of the region to be copied.

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Definitions:

Carbohydrate

Organic compounds, including sugars, starches, and cellulose, which serve as a major energy source for the body.

Lipid

A broad group of naturally occurring molecules including fats, oils, waxes, and certain vitamins, essential for energy storage and cell structure.

Nucleic Acid

Biomolecules essential to all known forms of life, including DNA and RNA, that carry genetic information necessary for growth, development, functioning, and reproduction.

Protein

Large biomolecules composed of amino acids, essential for various functions within organisms, including structural roles, enzymes, and cell signaling.