Examlex
Given the following information about a polypeptide
Hydrolysis of the polypeptide gives the following amino acids: Arg; Gly; Lys; Met(3) ; Phe(2) ;
Ser(2) ; Tyr.
Treatment of the polypeptide with chymotrypsin gives the following fragments: Met-Lys-Ser-Phe; Ser-Gly-Tyr; Met-Arg-Phe; Met.
Treatment of the polypeptide with cyanogen bromide gives the following fragments:
Ser-Gly-Tyr-[lactone]; Arg-Phe-[lactone]; Lys-Ser-Phe-[lactone].
Sanger degradation gives 2,4-DNP-Ser.
What is the primary structure of the original polypeptide?
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