You Have Made a Speci?c DNA Probe That Will Bind

question 65

Multiple Choice

You have made a speci?c DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. However, upon testing it out against a known Neisseria gonorrhoeae culture, you ?nd that it does not work. Which of the following is a possible explanation for this negative result?

• A) You forgot to incubate the test at the 75oC temperature required for hybridization.
• B) You forgot to label the probe sequence with a reporter molecule.
• C) You forgot to add the mRNA to the DNA probe sample.
• D) You forgot to digest the probe with restriction endonucleases.

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Definitions:

Winding Down Phase

The winding down phase is the period during which business operations are gradually ceased, and the final affairs of a business are concluded.

Collecting Assets

The process of identifying, gathering, and managing the assets of a debtor to satisfy claims during bankruptcy or liquidation.

Taking Inventory

The process of counting and listing items in stock, typically done periodically to control stock levels and assist in financial accounting.

Winding Up Process

The procedure of settling the accounts and liquidating the assets of a company for the purpose of distributing the net assets to shareholders and dissolving the business.