You Have Made a Speci?c DNA Probe That Will Bind

question 65

Multiple Choice

You have made a speci?c DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens. However, upon testing it out against a known Neisseria gonorrhoeae culture, you ?nd that it does not work. Which of the following is a possible explanation for this negative result?

• A) You forgot to incubate the test at the 75oC temperature required for hybridization.
• B) You forgot to label the probe sequence with a reporter molecule.
• C) You forgot to add the mRNA to the DNA probe sample.
• D) You forgot to digest the probe with restriction endonucleases.

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Definitions:

Freed Slaves

Individuals who were emancipated from slavery, often after legal acts or at the conclusion of conflicts such as the Civil War.

Racially Integrated

The process or state of combining people of different races in a community or institution.

Public Schools

Educational institutions funded and run by the government, providing free education to students as part of the public education system.

Black Suffrage

The right of African Americans to vote, a significant issue during the Reconstruction era in the United States, culminating in the passing of the 15th Amendment.