You Have Made a Smear of a Bacterial Culture and Have

question 92

Multiple Choice

You have made a smear of a bacterial culture and have performed the Gram stain on it. Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue- Purple of a gram-positive reaction, but instead are light purple. Your staining procedure was Performed correctly. What is your best explanation as to why the bacteria have stained this way?

• A) The specimen did not undergo heat ?xation before staining; therefore, the primary dye does not stick properly to the wall.
• B) These are mutant bacteria which cannot be stained like other bacteria.
• C) There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
• D) The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.

---

Definitions:

Adjusted Trial Balance

A financial statement that displays all balances of accounts after adjustments have been made, used to prepare final financial statements.

Unadjusted Trial Balance

A list of all the general ledger accounts and their balances at a specific point in time before any adjustments are made for the accounting period.

Columnar Working Paper

An accounting paper used for preparing financial statements or other reports, organized with columns to facilitate data entry and comparison.

Adjusted Trial Balance

A statement listing all accounts and their final balances after adjustments, used to verify the balance of debits and credits.