Why Have Frozen-Hydrated Particles Largely Replaced Negatively Stained Specimens for High

question 37

Multiple Choice

Why have frozen-hydrated particles largely replaced negatively stained specimens for high resolution cryo-EM studies?

• A)Frozen-hydrated particles are more likely to generate artifacts.
• B)Frozen-hydrated particles are much less likely to generate artifacts.
• C)Frozen-hydrated particles take positively charged stains more efficiently
• D)Frozen-hydrated particles are much better suited for the study of external features within the particle's structure.

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Definitions:

Capillary Walls

The thin, semi-permeable barriers of capillaries that allow for the exchange of oxygen, nutrients, and waste products between the blood and tissues.

Filtration

A process of separating suspended particles from fluids, gases, or other substances by passing them through a filter.

Osmosis

The diffusion of water across a semipermeable membrane such as a cell membrane.

Left Atrium

One of the four chambers of the heart, receiving oxygenated blood from the lungs and pumping it into the left ventricle.