A Gene Sequence Is Cut at a Restriction Site and DNA

question 88

Multiple Choice

A gene sequence is cut at a restriction site and DNA polymerase is then used to make the single-stranded regions of the sticky ends into double-stranded DNA. The ends of the altered DNA are then ligated back together. What is the effect on the gene sequence?

• A)Its amino acids remain constant
• B)The reading frame of the gene is destroyed.
• C)The gene is unchanged.
• D)The gene is transposed.
• E)The gene is inverted.

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Definitions:

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