You Are Working with Adult Mouse Fibroblast Cells

question 86

Multiple Choice

You are working with adult mouse fibroblast cells. The genes for transcription factors from pluripotent stem cells are transduced into the adult fibroblast cells as part of viral vectors in various combinations, and once inside the cells they are expressed. What results would you expect to obtain? (Select all correct choices)

• A)The fibroblasts die.
• B)A combination of transduced genes for only four specific transcription factors is sufficient to reprogram the fibroblasts into undifferentiated cells.
• C)A combination of transduced genes for only four specific transcription factors is sufficient to cause the fibroblasts to behave like pluripotent ES cells.
• D)A combination of transduced genes for only four specific transcription factors is sufficient to cause a terminal differentiation of the fibroblast cells.

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