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You Treat Some Cells with a Proteolytic Enzyme That Is

question 63

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You treat some cells with a proteolytic enzyme that is too large to penetrate the cell membrane (Set 1) . Another group of cells is made permeable to the enzyme before treatment with the enzyme (Set 2) . A third set of cells was not treated with the enzyme at all (controls) . Proteins are then extracted from the three different sets of cells and applied to an SDS-PAGE gel. Protein W migrates to the same distance on a gel of proteins from Set 1 and Set 2; Protein W migrates a shorter distance on gels of proteins extracted from the control group than on gels of proteins extracted from Set 1 and Set 2 treated cells. Protein X migrates to the same distance on a gel of proteins from control cells and the gels of the proteins from Set 1 and Set 2. Protein Y migrates a longer distance when extracted from Set 1 cells than does protein Y in the controls; Protein Y moves an even larger distance in the gel of the extracts from Set 2. Protein Z migrates the same distance on gels of proteins from the controls and the proteins extracted from Set 1, but it migrates a longer distance in extracts from Set 2 cells. Which protein is exposed only on the exterior of the cell?


Definitions:

Government Intervention

Actions taken by a government to influence its economy, which can include regulations, subsidies, and tariffs.

Market Economy

An economic system where supply and demand from private enterprises and consumers dictate the production of goods and services.

Invisible Hand

A term coined by Adam Smith to describe the self-regulating nature of the marketplace where individuals pursuing their own interest inadvertently benefit society at large.

Government Policies

Measures and regulations implemented by a government to influence economic, social, or administrative outcomes in the country.

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