An Enzyme Has a K_M of 20 µM and a V_max

question 9

Multiple Choice

An enzyme has a K_M of 20 µM and a V_max of 50 mmoles of product/minute/µg of enzyme. After exposure to an inhibitor and analysis on a Lineweaver-Burk plot the following values are obtained: -1/ K_M = - 0.05 liters/µmole and 1/ V_max = 0.04 (mmoles of product/minute/µg of enzyme) ^-1. What kind of inhibitor was used in the experiment and what data interpretation supports this conclusion?

• A)noncompetitive inhibitor because the K_M has remained the same and the V_max has decreased
• B)competitive inhibitor because the K_M has remained the same and the V_max has decreased
• C)noncompetitive inhibitor because the K_M has increased and the V_max has decreased
• D)competitive inhibitor because the K_M has increased and the V_max has decreased

---

Definitions:

Continuous Budget

A 12-month budget that rolls forward one month as the current month is completed.

Rolls Forward

A process in accounting or financial planning where data or balances are brought forward from a previous period to the current one.

Manufacturing Overhead Budget

A detailed plan showing the production costs, other than direct materials and direct labor, that will be incurred over a specified time period.

Non-cash Charges

Expenses reported on the income statement that do not involve a direct cash outflow, such as depreciation or amortization.