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An Enzyme Has a KM of 20 µM and a Vmax

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An enzyme has a KM of 20 µM and a Vmax of 50 mmoles of product/minute/µg of enzyme. After exposure to an inhibitor and analysis on a Lineweaver-Burk plot the following values are obtained: -1/ KM = - 0.03 liters/µmole and 1/ Vmax = 0.02 (mmoles of product/minute/µg of enzyme) -1. What kind of inhibitor was used in the experiment and what data interpretation supports this conclusion?

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Definitions:

Raw Materials

Basic materials that are used in the production process of goods, often transformed into finished products.

Fixed Overhead

Costs that do not vary with the level of production or sales, including rent, salaries, and insurance expenses.

Unfavorable Variance

A situation where actual results are worse than expected results, often indicating higher costs or lower revenues than planned.

Favorable Variance

A financial term indicating that actual costs were lower than the planned or standard costs in a budget.

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