You Are Working with an Enzyme Altase That You Denature

question 51

Multiple Choice

You are working with an enzyme altase that you denature in the presence of urea. If altase were denatured no further by the addition of mercaptoethanol, what would that suggest to you about the enzyme?

• A)The enzyme probably contained no positively charged amino acids since these are neutralized by mercaptoethanol.
• B)The enzyme probably contained no acidic amino acids since these are neutralized by mercaptoethanol.
• C)The enzyme probably contained no disulfide linkages since mercaptoethanol breaks such linkages.
• D)Mercaptoethanol and urea denature enzymes in the same manner, so the observation lacks significance.

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Definitions:

Standard Error

A statistical measure that describes the accuracy with which a sample mean estimates the population mean.

Regression Line

A line of best fit that describes the linear relationship between an independent variable and a dependent variable.

Least-Squares Regression

A statistical method that determines the best-fitting line for a set of data by minimizing the sum of the squares of the vertical distances of the points from the line.

Slope

The measure of the steepness or incline of a line, indicating the rate at which values increase or decrease.