Use This Information to Answer the Following Questions

question 5

Multiple Choice

Use this information to answer the following questions.
Affinity chromatography is a method that can be used to purify cell-surface receptors, while they retain their hormone-binding ability. A ligand (hormone) for a receptor of interest is chemically linked to polystyrene beads. A solubilized preparation of membrane proteins is passed over a column containing these beads. Only the receptor binds to the beads.
-When an excess of the ligand (hormone) is poured through the column after the receptor binding step, what do you expect will occur?

• A) The ligand will attach to those beads that have the receptor and remain on the column.
• B) The ligand will cause the receptor to be displaced from the beads and eluted out.
• C) The ligand will attach to the bead instead of the receptor.
• D) The ligand will cause the bead to lose its affinity by changing shape.
• E) The reaction will cause a pH change due to electron transfer.

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