A Researcher Has Used in Vitro Mutagenesis to Mutate a Cloned

question 43

Multiple Choice

A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?

• A) recombination resulting in replacement of the wild type with the mutated gene
• B) use of a microarray to verify continued expression of the original gene
• C) replication of the cloned gene using a bacterial plasmid
• D) transcription of the cloned gene using a BAC
• E) attachment of the mutated gene to an existing mRNA to be translated

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