Use the Following Information to Answer the Next Few Questions

question 34

Multiple Choice

Use the following information to answer the next few questions.
CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes overexpression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early.
-Which of the following would be a reasonably efficient technique for confirming the diagnosis of CML?

• A) searching for the number of telomeric sequences on chromosome 22
• B) looking for a Ph' chromosome in a peripheral blood smear
• C) enzyme assay for thymine kinase activity
• D) FISH study to determine the chromosomal location of all chromosome 22 fragments
• E) identification of the disease phenotype in review of the patient's records