Assume That You Are Trying to Insert a Gene into a Plasmid

question 2

Multiple Choice

Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

• A) insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid.
• B) cut the plasmid with restriction enzyme X and insert the fragments cut with restriction enzyme Y into the plasmid.
• C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
• D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.
• E) cut the plasmid with restriction enzyme X and then insert the gene into the plasmid.

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Definitions:

Short-Term Memory

The capability to maintain a small volume of information in a condition of ready availability for a short time.

Short-Term Memory

The capacity for holding a small amount of information in an active, readily available state for a short period of time.

Magic Number

A term often used to refer to the number 7±2, which psychology suggests is the span of working memory, or the maximum number of items an individual can maintain in cognizant thought at once.

Chunking

A memory technique that organizes information into manageable units or chunks, making it easier to remember.