A Principal Problem with Inserting an Unmodified Mammalian Gene into a BAC

question 65

Multiple Choice

A principal problem with inserting an unmodified mammalian gene into a BAC, and then getting that gene expressed in bacteria, is that

• A) prokaryotes use a different genetic code from that of eukaryotes.
• B) bacteria translate polycistronic messages only.
• C) bacteria cannot remove eukaryotic introns.
• D) bacterial RNA polymerase cannot make RNA complementary to mammalian DNA.
• E) bacterial DNA is not found in a membrane-bounded nucleus and is therefore incompatible with mammalian DNA.

---

Definitions:

Back Pain

Discomfort or aches in the lower, middle, or upper back, often stemming from the muscles, nerves, bones, joints, or other structures in the spine.

Neural Gate

A theoretical mechanism within the spinal cord that regulates the passage of pain signals to the brain.

Small Nerve Fibers

Nerve fibers of small diameter that are responsible for transmitting pain and temperature sensations.

Spinal Cord

A long, thin, tubular structure made up of nervous tissue, which extends from the brainstem down the back, and transmits neural signals between the brain and the body.