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To accurately isolate cause and effect,experimenters should use
Melatonin
A hormone produced by the pineal gland that regulates sleep-wake cycles and is often used to treat sleep disorders.
REM Sleep
Rapid Eye Movement (REM) sleep, a unique phase of sleep involving vivid dreams, and is thought to play a role in learning and memory.
N3 Sleep
The third stage of non-REM sleep, characterized by deep sleep and the slowest brain waves during sleep, essential for restorative sleep.
N2 Sleep
A stage of sleep associated with the first instances of true sleep spindles and K-complexes, marking deeper sleep than N1.
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