DNA Sequencing by Synthesis Is a Powerful Contemporary Technique That

question 33

Multiple Choice

DNA sequencing by synthesis is a powerful contemporary technique that allows the generation of up to 600 gigabases (Gb) of sequence per run. Which process is part of sequencing by synthesis?

• A) The sequencing products are separated by polyacrylamide gel electrophoresis.
• B) [32P]-DNA fragments are developed using X-ray film or phosphoimaging.
• C) The release of pyrophosphate during the incorporation of dNTPs into a growing DNA chain is detected by a complex reaction system that emits light from oxyluciferin.
• D) About one thousand identical copies of each individual template DNA form a tight cluster (at the mm-level) on the surface of a flow cell prior to sequencing.
• E) Whole large DNA molecules, such as plasmids or BACs, are used as templates.

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Definitions:

Universal Needs

Fundamental needs that are commonly considered essential for all humans, such as food, water, shelter, and safety.

Aesthetic Need

The human desire for beauty and visually pleasing experiences in their surroundings.

Sensation Seeker

A person who seeks out new and different sensations, experiences, and risks to achieve a heightened sense of excitement and adventure.

Arousal Theory

Arousal Theory suggests that people are motivated to engage in behaviors that help them maintain an optimal level of arousal, balancing between stimulation and relaxation.