Which of the Following Describes the Process of Obtaining RNA

question 18

Multiple Choice

Which of the following describes the process of obtaining RNA sequence information?

• A) Proteins are isolated and mass spec is used. Once the proteins are known, the RNA that coded for them can be determined.
• B) RNA from a particular growth condition is isolated and converted to cDNA; DNA adapters are added, allowing high-throughput sequencing to be carried out. The genome position of the sequence obtained is then determined using computer software.
• C) A DNA microarray is used to find the RNA sequences expressed at that time.
• D) One examines the tRNA and rRNA in a given culture in order to study various physiological conditions.
• E) One determines the RNA sequence of every gene in the genome and then determines if it is present in a given culture.

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Definitions:

Nitrogen

A chemical element with symbol N and atomic number 7, essential for all living organisms as a constituent of proteins.

Synergistic Interaction

A situation where two or more substances or actions combine to produce a total effect greater than the sum of their individual effects.

Isotopes

Variants of a particular chemical element that differ in neutron number, and consequently in nucleon number, but have the same number of protons in their atomic nuclei.

Neutrons

Subatomic particles located within the nucleus of an atom, having no electric charge and a mass slightly larger than that of protons.