Which of the Following Experimental Procedures Is NOT Used in ChIP-Seq

question 63

Multiple Choice

Which of the following experimental procedures is NOT used in ChIP-seq?

• A) DNA-protein complexes are cross-linked.
• B) DNA is extracted and sheared.
• C) Bead-bound antibodies to transcription factors are used to precipitate transcription factors cross-linked with DNA.
• D) The bead-bound antibodies are precipitated using a secondary antibody.
• E) Transcription factors are released from DNA, amplified and identified by sequencing.

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Definitions:

Viral Surface

The exterior part of a virus particle that interacts with a host's cellular mechanisms, often through specific proteins or receptors.

Host Cell

A living cell in which a virus or parasite invades and multiplies, often causing harm to the host.

Sirenians

Aquatic, herbivorous mammals that include species such as manatees and dugongs, known for their slow-moving nature and paddle-like flippers.

Homologous Trait

Features in different species that are similar because of common ancestry, indicating evolutionary relationships.