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In the Yeast Two-Hybrid Analysis Technique, in Vivo Protein-Protein Interactions

question 55

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In the yeast two-hybrid analysis technique, in vivo protein-protein interactions are explored. The "bait" and "prey" proteins are translationally fused to the cleaved activation and DNA-binding domains of the GAL4 transcription factor. What is the target gene of GAL4?

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Definitions:

Superego

In Freudian psychoanalysis, the part of the personality that acts as a moral center, imposing the morals and standards learned from parents and society.

Phenylketonuria

A genetic disorder characterized by an inability to metabolize the amino acid phenylalanine, leading to its accumulation and potentially severe neurological damage if untreated.

Huntington Disease

Hereditary disease characterized by chorea (ceaseless involuntary jerky movements) and progressive dementia, ending in death.

Tay-Sachs Disease

Inherited birth defect caused by the loss of genes that encode the enzyme necessary for breaking down certain fatty substances; appears 4 to 6 months after birth and results in intellectual disability, physical changes, and death by about age 5.

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