A Standardized Inoculum of Bacteria Is Used in All Microbiology

question 47

Multiple Choice

A standardized inoculum of bacteria is used in all microbiology labs when performing a Kirby-Bauer assay. This standard allows for a visual turbidity reading to achieve reproducibility in the number of bacteria inoculated on a plate. This is most important because

• A) multiple antibiotics testing requires that bacteria be spread evenly around the plate.
• B) the MIC value can only be achieved by serial dilutions.
• C) the number of organisms placed on an agar surface is inversely proportional to the size of the zone of inhibition.
• D) bacterial counts need to be the same across all labs, since incubation times can differ.
• E) bacterial counts need to be the same across all labs, since plate sizes in this assay can differ.

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