Use the Following Information to Answer the Question

question 4

Multiple Choice

Use the following information to answer the question.
"The native structure of hemoglobin (Hb) comprises of two α and two β subunits, each of which carries a heme group. There appear to be no previous studies that report the in-vitro folding and assembly of Hb from highly unfolded α and β globin in a 'one-pot' reaction. One difficulty that has to be overcome for studies of this kind is the tendency of Hb to aggregate during refolding. This work demonstrates that denaturation of Hb in 40% acetonitrile at pH 10.0 is reversible." (J Am Soc Mass Spectrum 2007, 18, 8-16)
Hemoglobin, when subjected to 40% acetonitrile at pH 10.0, loses its quaternary structure, which means the ________.

• A) four α and β polypeptides dissociate
• B) peptide bonds between amino acids break
• C) α and β polypeptides lose their 3-D structure
• D) four α and β polypeptides dissociate, peptide bonds between amino acids, and α and β polypeptides lose their 3-D structure

---

Definitions:

Nonprogrammed Decision

pertains to decisions made in response to situations that are unique, are poorly defined and largely unstructured, requiring a custom-tailored solution.

Unique Problem

Refers to a problem that is distinct and different in its characteristics, not fitting typical patterns or previous experiences.

Programmed Decisions

Decisions that are routine and repetitive, for which established rules or procedures can be developed and applied.

Structured Problems

Structured problems are well-defined, clear, and straightforward issues that can be solved through recognized procedures and methods.