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The Following Question Refers to the Figure

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The following question refers to the figure.
In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population.
The following question refers to the figure. In this eight-year experiment, 12 populations of E. coli, each begun from a single cell, were grown in low-glucose conditions for 20,000 generations. Each culture was introduced to fresh growth medium every 24 hours. Occasionally, samples were removed from the populations, and their fitness in low-glucose conditions was tested against that of members sampled from the ancestral (common ancestor) E. coli population.   If the experimental population of E. coli lacks an F factor or F plasmid, and if bacteriophages are excluded from the bacterial cultures, then beneficial mutations might be transmitted horizontally to other E. coli cells via ________. A)  sex pili B)  transduction C)  conjugation D)  transformation
If the experimental population of E. coli lacks an F factor or F plasmid, and if bacteriophages are excluded from the bacterial cultures, then beneficial mutations might be transmitted horizontally to other E. coli cells via ________.


Definitions:

Alternation of Generations

A type of life cycle characteristic of plants and a few algae and fungi in which they spend part of their life in a multicellular n gametophyte stage and part in a multicellular 2n sporophyte stage.

Gametophyte

The reproductive stage in the life cycle of plants and algae where gametes, the cells responsible for reproduction, are created.

Sporophyte

The diploid multicellular stage in the life cycle of plants and algae that produces spores through meiotic division.

Diploid

Refers to cells that contain two complete sets of chromosomes, one from each parent.

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