You Are Trying to Design a Protein That Will Be

question 18

Essay

You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system.

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Definitions:

V

Typically represents voltage in electrical contexts, indicating the electrical potential difference between two points.

Cycle

In electrical terms, a cycle refers to one complete oscillation or wave of alternating current or voltage.

Frequency

The rate at which an alternating current or a repetitive electronic signal oscillates, typically measured in hertz (Hz).

Hertz

The unit of frequency in the International System of Units (SI), equal to one cycle per second.