You Experimentally Change the DNA Sequence Directly Upstream of a Start

question 10

Multiple Choice

You experimentally change the DNA sequence directly upstream of a start codon of an operon in E. coli to investigate the function of this region of DNA. Analysis reveals that after the change the same amount of mRNA is made from the operon, but there are very few proteins made from the operon. What is the most likely function of the DNA sequence that you changed?

• A) The DNA sequence likely functions as a ribosome-binding site.
• B) The DNA sequence likely functions as a promoter.
• C) The DNA sequence likely functions as a termination sequence.
• D) The DNA sequence likely functions as in transcriptional regulation.

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