You Are Trying to Design a Protein That Will Be

question 18

Essay

You are trying to design a protein that will be expressed in Escherichia coli and secreted outside of the cell for purification and use as a pharmaceutical. E. coli is a gram-negative cell and the protein folds after it has exited the cell. Which secretion system would work best for this project? Support your answer with evidence based on the properties of E. coli, the protein, and the secretion system.

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Definitions:

Eibl-Eibesfeldt

Refers to Irenäus Eibl-Eibesfeldt, an ethologist known for his work in human behavior and evolutionary biology.

Human Facial Expressions

The visible changes in the face that convey an individual's emotional state, intentions, or social communication.

Basic Emotions

Fundamental feelings that are universal across human cultures, such as happiness, sadness, fear, disgust, anger, and surprise.

Neurotransmitter

Biological chemicals that transmit signals across synapses from one neuron to another in the brain.