What Is the Purpose of Synthesizing a Negative Strand RNA

question 12

Multiple Choice

What is the purpose of synthesizing a negative strand RNA in positive stranded ssRNA viruses?

• A) enable rolling circle amplification of the genome, which requires both strands of RNA
• B) enable transcription of genes occurring on both the negative and positive strands of the genome, such as overlapping genes
• C) proofreading of the genome to minimize mutations generated by the polymerase being passed onto virion progeny
• D) to serve as the complementary template sequence in genome amplification of the positive strand

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