At Which Time Period(s) During PCR Thermocycling Is/are Hottest in Temperature

question 31

Multiple Choice

At which time period(s) during PCR thermocycling is/are hottest in temperature?

• A) during DNA denaturation
• B) during primer annealing
• C) during primer extension/elongation
• D) Both the first and last cycles are hotter in temperature than all other cycles.

---

Definitions:

Perspective Taking

The ability to understand and consider someone else's point of view or mental state.

Selman's Stages

A framework proposed by Robert Selman outlining the stages of social perspective-taking development in children, reflecting how they understand others' thoughts and feelings.

Self-Reflective

Having the ability or tendency to think about and analyze one’s own thoughts, feelings, and behaviors.

Math Problems

Challenges or exercises requiring counting, calculation, and logical reasoning skills, often used in educational settings to develop numerical and critical thinking abilities.