Some Proteins Expressed at High Levels Form Inclusions That Are

question 82

Multiple Choice

Some proteins expressed at high levels form inclusions that are relatively insoluble. What is the most effective way to facilitate purification of these proteins?

• A) Use a reporter gene to locate the inclusion.
• B) Decrease the number of insertions in the vector.
• C) Produce the protein as a fusion protein.
• D) Switch to an expression host with a larger intracellular volume.

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Definitions:

Calcium

A chemical element, symbol Ca, essential for living organisms, particularly in cell physiology and bone formation.

Humerus

The humerus is the long bone in the upper arm, extending from the shoulder to the elbow.

Tibia

A large bone located in the lower leg, between the knee and the ankle, parallel to the fibula.

Ulna

One of the two long bones in the forearm, located on the side opposite the thumb and running parallel to the radius.