Some Proteins Expressed at High Levels Form Inclusions That Are

question 82

Multiple Choice

Some proteins expressed at high levels form inclusions that are relatively insoluble. What is the most effective way to facilitate purification of these proteins?

• A) Use a reporter gene to locate the inclusion.
• B) Decrease the number of insertions in the vector.
• C) Produce the protein as a fusion protein.
• D) Switch to an expression host with a larger intracellular volume.