You Are Culturing a New Bacterium in the Laboratory

question 32

Multiple Choice

You are culturing a new bacterium in the laboratory. You cannot find any glucose it the lab when you are making growth medium, so you decide to use honey instead. You add twice as much honey than is called for in the medium recipe, because you want to give the organism the best opportunity or growth. To your surprise, the organism does not grow on your modified medium. Select the most likely explanation.

• A) The growth medium became hypotonic with the addition of so much honey; the bacteria underwent plasmolysis and died.
• B) The growth medium became hypertonic with the addition of so much honey; the bacteria underwent plasmolysis and died.
• C) The growth medium became halophilic with the addition of so much honey; the bacteria underwent plasmolysis and died.
• D) The growth medium became hypertonic with the addition of so much honey; the bacteria underwent osmotic lysis and died.
• E) The growth medium became hypotonic with the addition of so much honey; the bacteria underwent simple diffusion and burst.

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Definitions:

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Workers who are employed by an organization in positions that may require them to move between different countries or work across national boundaries.

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Employees from an organization's founding country working at its operations in another country, often in managerial or technical positions.

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