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A Cluster of Cases of Legionellosis Have Occurred in Your

question 65

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A cluster of cases of legionellosis have occurred in your neighborhood. People with the illness have cough, fever, shortness of breath, chest pain and sometimes diarrhea. The illness is caused by the bacterium Legionella pneumophila, a facultative intracellular parasite that survives in certain ameba. The organism has an absolute requirement for L-cysteine.
-A modification of the BCYE medium used to culture L. pneumophila is BCYE with DGVP. This medium contains glycine, polymixin B (an antifungal agent) , and and vancomycin (antibiotic) , as well as two dyes (bromocreson purple and bromthymol blue) that facilitate identification of the organism based on colony color. What type of medium is BCYE with DGVP?


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