A Mutation in E

question 7

Multiple Choice

A mutation in E. coli results in the loss of both restriction endonucleases and modification enzymes. Would you expect any difference in the frequency of gene transfer via transduction FROM Salmonella INTO this E. coli strain?

• A) No-since the Salmonella strain is normal, the rate of production of transducing virus particles would still be the same, resulting in the same frequency of gene transfer.
• B) Yes-the loss of the restriction endonucleases would leave the recipient E. coli unable to break down "invading"' viral DNA from the transducing phage. This would lead to higher rates of successful transduction.
• C) Yes-the loss of the modification enzymes would leave the recipient E. coli unable to tag its own DNA as "self," leaving the viral DNA untagged and recognizable as "foreign," and targeted for destruction. This would lead to higher rates of successful transduction.
• D) No-transduction efficiency isn't affected by either restriction endonucleases or modification enzymes, so there'd be no effect on the overall rate.
• E) Yes-the loss of the restriction endonucleases would leave the recipient E. coli unable to break down "invading" viral DNA from the transducing phage, AND the loss of the modification enzymes would leave the recipient E. coli unable to tag its own DNA as "self," leaving the viral DNA untagged and recognizable as "foreign," and targeted for destruction. Together, these would lead to higher rates of successful transduction.

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Definitions:

Health Belief Model

A psychological model that explains and predicts health behaviors by focusing on the attitudes and beliefs of individuals.

Nondirective

An approach in therapy and counseling where the therapist provides support without leading the client towards specific solutions, empowering them to make their own decisions.

Patient-Centered

An approach to healthcare that respects and responds to individual patient preferences, needs, and values.

Motivational Interviewing

A counseling approach that helps individuals find the motivation to make positive decisions and accomplish established goals.