PCR Generates a DNA Fragment of a Distinct Size Even

question 14

Multiple Choice

PCR generates a DNA fragment of a distinct size even when the whole viral chromosome is used as a template. What determines the boundaries of the amplified fragment?

• A) The concentration of one particular deoxynucleotide in the reaction.
• B) The length of time of the elongation step in each cycle.
• C) The position of a termination sequence, which causes the Taq polymerase to fall off the template.
• D) The sites on the template DNA to which the specific primers used in the reaction anneal.
• E) The temperature at which the elongation step in each cycle is carried out.

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Definitions:

GABA

Gamma-Aminobutyric Acid, an inhibitory neurotransmitter in the brain that helps regulate neuronal excitability and plays a key role in reducing anxiety and promoting relaxation.

Two-Factor Theory

The most influential theory of fear and phobias during the 1960s and 1970s. The model proposed that fears are acquired by classical conditioning, but maintained by operant conditioning.

Little Albert

The subject of a famous psychology experiment by John B. Watson which demonstrated that emotional responses could be conditioned in humans.

Little Hans

A case study by Sigmund Freud involving the analysis of a young boy's fear of horses, which Freud interpreted in terms of the Oedipus complex.