When DNA Probes Are Used to Identify Bacterial DNA Similarities

question 5

Multiple Choice

When DNA probes are used to identify bacterial DNA similarities by hybridization, the probe DNA is heated and the template DNA is treated to separate the two strands. Why would the probe DNA be heated?

• A) This is the only way to properly label the probe DNA.
• B) The probe may contain parts that are double-stranded. Heating it up breaks the hydrogen bonds holding the strands together, creating single-stranded sequences that hybridize with their complementary targets.
• C) Heating it up activates the tag on the probe DNA before it hybridizes to the bacterial template DNA.
• D) DNA hybridization can only take place at high temperatures, so all the DNA must be heated up prior to hybridization.
• E) The probe may contain many parts that are single-stranded. Heating it up creates hydrogen bonds that hold the probe tightly to its complementary target sequences.

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Definitions:

Ending Inventory

The total value of goods available for sale at the end of an accounting period, calculated by adding new purchases to the starting inventory and subtracting goods sold.

Inventory Valuation Methods

Techniques used to calculate the cost of goods sold and ending inventory, such as FIFO (First-In, First-Out), LIFO (Last-In, First-Out), and average cost methods.

Gross Profit Method

An accounting technique used to estimate the amount of ending inventory and cost of goods sold, based on the gross profit margin.

Gross Profit Ratio

A financial metric that indicates the proportion of money left over from revenues after deducting the cost of goods sold.