You Are Sent Three Clinical Samples and Are Asked to Determine

question 55

Multiple Choice

You are sent three clinical samples and are asked to determine whether they are the same strain. You perform standard identification techniques but cannot come to a conclusion. You decide to do an RFLP test. You digest the genomes of the organisms with the same restriction enzyme and run a gel. Each organism yields 5 bands on the gel, of the following sizes: 
Organism A: 3 kb, 7 kb, 11 kb; 14 kb, 21 kb
Organism B: 3 kb, 7 kb, 2 kb, 8 kb, 11 kb
Organism C: 11 kb, 3 kb, 14 kb, 7 kb; 21 kb
What conclusion(s) can you make from this result?

• A) Organisms A and B are likely the same strain but are different from organism C.
• B) Organisms B and C are likely the same strain but are different from organism A.
• C) Organisms A and C are likely the same strain but are different from organism B.
• D) The organisms are all likely the same strain.
• E) The organisms are all likely different strains.

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