Suppose That You Have Cloned a Genomic Library and Know

question 26

Multiple Choice

Suppose that you have cloned a genomic library and know that the bacteria have foreign DNA fragments inserted into the cloning vector. Also suppose that you need to identify the specific bacteria that have the gene coding for protein X. How can these bacteria be located?

• A) by restriction endonuclease treatment of DNA from some of the bacterial colonies
• B) by DNA hybridization of the different colonies
• C) by agarose gel electrophoresis of DNA isolated from the bacterial colonies
• D) by PCR of the same bacterial mixture that was spread on agarose plates